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KMID : 0900920030270040275
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Korean journal of Animal Reproduction
2003 Volume.27 No. 4 p.275 ~ p.280
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Effect of Cysteamine on In Vitro Maturation, Fertilization and Culture of Porcine Oocytes
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Yi Y.-J.
Kim M.-Y.
Lee S.-H.
Min T.-S.
Jin D.-I.
Park C.-S.
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Abstract
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This study was carried out to investigate the effect of cysteamine addition during in vitro maturation, fertilization and culture of porcine oocytes. Oocytes were matured for the first 22 h in mTCM -199 media supplemented with or without 150 muM cysteamine. They then were matured for an additional 22 h in mTCM-199 media without hormones supplemented with or without 150 muM cysteamine. When cumulus-oocyte complexes (COCs) were matured in the mTCM-199 media supplemented with cysteamine, the rates of GVBD and maturation (metaphase II) were enhanced as compared to the media without the addition of cysteamine. Also, when COCs were matured in the mTCM-199 media supplemented with cysteamine, the rates of sperm penetration, male pronucleus formation, cleavage and blastocyst formation after in vitro fertilization were enhanced as compared to the media without the addition of cysteamine. In conclusion, it was suggested that oocytes matured for the first 22 h in mTCM-199 media supplemented with 150 muM cysteamine increased the rates of metaphase II, sperm penetration, male pronucleus and blastocyst formation were higher as compared to the media without addition of cysteamine.
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KEYWORD
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Maturation, Cysteamine, Male pronucleus, Blastocyst
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